Recombinant Human IL-10 Protein

重组人IL-10蛋白 | 重组人白细胞介素-10蛋白

CSIF | CSIFMGC126450 | Cytokine synthesis inhibitory factor | GVHDS | IL10 | IL-10 | IL10A | IL-10MGC126451 | interleukin 10 | interleukin-10 | TGIF

rp156176-10μg

10μg

无动物源

≥90%(SDS-PAGE)

Interleukin 10, also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL‑10 family of alpha ‑helical cytokines that also includes IL‑19, IL‑20, IL‑22, IL‑24, and IL‑26/AK155. IL‑10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts. Mature human IL‑10 shares 72% ‑ 86% amino acid sequence identity with bovine, canine, equine, feline, mouse, ovine, porcine, and rat IL‑10. Whereas human IL‑10 is active on mouse cells, mouse IL‑10 does not act on human cells. IL‑10 is a 178 amino acid molecule that contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently associated homodimer. The IL‑10 dimer binds to two IL‑10 R alpha /IL‑10 R1 chains, resulting in recruitment of two IL‑10 R beta /IL‑10 R2 chains and activation of a signaling cascade involving JAK1, TYK2, and STAT3. IL‑10 R beta does not bind IL‑10 by itself but is required for signal transduction. IL‑10 R beta also associates with IL‑20 R alpha, IL‑22 R alpha, or IL‑28 R alpha to form the receptor complexes for IL‑22, IL‑26, IL‑28, and IL‑29. IL‑10 is a critical molecule in the control of viral infections and allergic and autoimmune inflammation. It promotes phagocytic uptake and Th2 responses but suppresses antigen presentation and Th1 proinflammatory responses.

Immobilized Recombinant Human IL-10 Protein (rp156176) at 5.0 μg/mL can bind Recombinant Human IL-10 R alpha Protein (rp147368) with the EC50 of 323.7 ng/mL. Immobilized Recombinant Human IL-10 Protein (rp156176) at 5.0 μg/mL can bind IL-10 Mouse mAb (Ab109811) with the EC50 of 30.3 ng/mL.

重组表达

19.5 kDa

C-His

17.4 kDa, under reducing conditions; 17.7 & 16.3 kDa, under non-reducing conditions.

HEK293 Accession #: P22301 | HEK293

<1.0 EU/μg

人(Human)

19-178 aa

SPGQGTQSENSCTHFPGNLPNMLRDLRDAFSRVKTFFQMKDQLDNLLLKESLLEDFKGYLGCQALSEMIQFYLEEVMPQAENQDPDIKAHVNSLGENLKTLRLRLRRCHRFLPCENKSKAVEQVKNAFNKLQEKGIYKAMSEFDIFINYIEAYMTMKIRNHHHHHH​

Yes

Yes