Recombinant Human CD34 Protein

重组人CD34蛋白 | 重组人 CD34 蛋白

CD34 | CD34 antigen | CD34 molecule | CD34_HUMAN | Cluster designation 34 | cluster of differentiation 34 | Hematopoietic progenitor cell antigen CD34 | HPCA1 | Mucosialin | OTTHUMP00000034733 | OTTHUMP00000034734

rp156652-50μg

50μg

无动物源

≥95%(SDS-PAGE)

>95% (SDS-PAGE)

Purity:>95%, by SDS-PAGE visualized with Coomassie® Blue Staining Description: CD34 is a 115 kDa glycosylated type I transmembrane protein; it was discovered as a hematopoietic cell-surface antigen. Human CD34 cDNA encodes a 385 amino acid (aa) precursor that contains a 31 aa signal sequence, a 259 aa extracellular domain (ECD), a 21 aa transmembrane sequence, and a 74 aa cytoplasmic domain. Within the ECD, human CD34 shares 55% and 52% aa sequence identity with mouse and rat CD34, respectively. This single-pass sialomucin‑like transmembrane protein is heavily glycosylated and phosphorylated by Protein Kinase C (PKC). CD34 is found on multipotent precursors, bone marrow stromal cells, embryonic fibroblasts, vascular endothelia, as well as some populations of mesenchymal stem cells, and tumor cell lines, and it is a common marker for diverse progenitors. CD34 is involved in the adhesion of stem cells to the bone marrow extracellular matrix or to stromal cells.

Measured by its binding ability in a functional ELISA. Immobilized human CD34 (rp156652) at 2.0 μg/mL can bind Mouse CD34 mAb (Ab094638) with the EC₅₀ of 31.41 ng/mL.

重组表达

27.5 kDa

C-His

96.5-131.2 kDa, under reducing conditions; 96.5-131.2 kDa, under non-reducing condition

HEK293 Accession #: P28906 | HEK293

<1.0 EU/μg

人(Human)

1-290 aa

MLVRRGARAGPRMPRGWTALCLLSLLPSGFMSLDNNGTATPELPTQGTFSNVSTNVSYQETTTPSTLGSTSLHPVSQHGNEATTNITETTVKFTSTSVITSVYGNTNSSVQSQTSVISTVFTTPANVSTPETTLKPSLSPGNVSDLSTTSTSLATSPTKPYTSSSPILSDIKAEIKCSGIREVKLTQGICLEQNKTSSCAEFKKDRGEGLARVLCGEEQADADAGAQVCSLLLAQSEVRPQCLLLVLANRTEISSKLQLMKKHQSDLKKLGILDFTEQDVASHQSYSQKTHHHHHH

Yes

Yes