Recombinant Human VEGF 165 GMP Protein

重组人血管内皮生长因子VEGF165 | 重组人 VEGF 165 GMP 蛋白

MVCD1 | VAS | vascular endothelial growth factor A | Vascular permeability factor | Vasculotropin | VEGF | VEGFA | VEGF-A | VEGFMGC70609 | VPF | VPFvascular endothelial growth factor | L-VEGF

rp166634-GMP-50μg

50μg

无动物源

≥97%(SDS-PAGE&SEC-HPLC)

血管生成、血管生成和内皮细胞生长过程中的活跃生长因子。诱导内皮细胞增殖、促进细胞迁移、抑制细胞凋亡并诱导血管通透。与 FLT1/VEGFR1 和 KDR/VEGFR2 受体、硫酸肝素和肝素结合。NRP1/Neuropilin-1 与 VEGF-165 和 VEGF-145 异构体结合。异构体 VEGF165B 可与 KDR 结合，但不会激活下游信号通路，不会激活血管生成并抑制肿瘤生长。

Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED50 for this effect is 1.50-12.0 ng/mL. The specific activity of recombinant human VEGF165 is > 8.0 x 10^5 units/mg, which is calibrated against the human VEGF165 WHO standard (NIBSC code: 02/286).

重组表达

19.2 kDa (monomer)

No tag

19-21 kDa, under reducing conditions

E. coli Accession #: NP_001165097.1 | E. coli

<0.01 EU/μg

人(Human)

27-191 aa

APMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQENPCGPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR

Yes

Yes

Met-Ala27-Pro-Met-Ala-Glu-Gly-Gly-Gly-Gln Pro28-Met-Ala-Glu-Gly-Gly-Gly-Gln-Asn-His