Recombinant Human HGF GMP Protein

重组人HGF蛋白 | 重组人肝细胞生长因子蛋白 | 重组人HGF GMP蛋白

Hepatocyte Growth Factor | deafness, autosomal recessive 39 | DFNB39 | EC 3.4.21 | EC 3.4.21.7 | fibroblast-derived tumor cytotoxic factor | F-TCF | hepatocyte growth factor (hepapoietin A | scatter factor) | Hepatopoeitin-A | Hepatopoietin A | HGF | HGFB

rp168049-GMP-25μg

25μg

无动物源

≥95%(SDS-PAGE&SEC-HPLC)

成熟实质肝细胞的强效有丝分裂原，似乎是一种肝营养因子，也是多种组织和细胞类型的生长因子。通过与受体酪氨酸激酶 MET 结合并促进其二聚化，激活 MET 的配体。在 TMPRSS13 分裂和激活后激活 MAPK 信号。

Measured by its ability to induce IL-11 secretion by Saos‑2 human osteosarcoma cells. The ED50 for this effect is equal or less than 4.00 ng/mL. The specific activity of recombinant human HGF is >1.0 x 10^6 IU/mg, which is calibrated against the human HGF WHO International Standard (NIBSC code: 96/564).

重组表达

α chain: 53.7 kDa, β chain: 26 kDa

71-85 kDa, non-reducing conditions. 56-65 kDa and 30-40 kDa, reducing conditions.

HEK293 Accession #: P14210-1 | HEK293

<0.1 EU/μg

人(Human)

32-728 aa (D384N, D416N, L622S, H645R, V678I)

QRKRRNTIHEFKKSAKTTLIKIDPALKIKTKKVNTADQCANRCTRNKGLPFTCKAFVFDKARKQCLWFPFNSMSSGVKKEFGHEFDLYENKDYIRNCIIGKGRSYKGTVSITKSGIKCQPWSSMIPHEHSFLPSSYRGKDLQENYCRNPRGEEGGPWCFTSNPEVRYEVCDIPQCSEVECMTCNGESYRGLMDHTESGKICQRWDHQTPHRHKFLPERYPDKGFDDNYCRNPDGQPRPWCYTLDPHTRWEYCAIKTCADNTMNDTDVPLETTECIQGQGEGYRGTVNTIWNGIPCQRWDSQYPHEHDMTPENFKCKDLRENYCRNPDGSESPWCFTTDPNIRVGYCSQIPNCNMSHGQDCYRGNGKNYMGNLSQTRSGLTCSMWNKNMEDLHRHIFWEPDASKLNENYCRNPDDDAHGPWCYTGNPLIPWDYCPISRCEGDTTPTIVNLDHPVISCAKTKQLRVVNGIPTRTNIGWMVSLRYRNKHICGGSLIKESWVLTARQCFPSRDLKDYEAWLGIHDVHGRGDEKCKQVLNVSQLVYGPEGSDLVLMKLARPAVLDDFVSTIDLPNYGCTIPEKTSCSVYGWGYTGSINYDGLLRVAHLYIMGNEKCSQRHRGKVTLNESEICAGAEKIGSGPCEGDYGGPLICEQHKMRMVLGVIVPGRGCAIPNRPGIFVRVAYYAKWIHKIILTYKVPQS

Yes

Yes

Gln32-Arg-Lys-Arg-Arg-Asn-Thr-Ile-His-Glu (α-chain) Val495-Val-Asn-Gly-Ile-Pro-Thr-Arg-Thr-Asn (β-chain)