Recombinant Human GDNF GMP Protein

重组人GDNF蛋白 | 重组人胶质细胞系来源神经营养因子蛋白 | 重组人 GDNF GMP 蛋白

Glial Cell line-derived Growth Factor | Astrocyte-derived trophic factor | ATF | ATF1 | ATF2 | GDNF | glial cell derived neurotrophic factor | glial cell line derived neurotrophic factor | glial cell line-derived neurotrophic factor | HFB1-GDNF | HGDNF |

rp168357-GMP-10μg

10μg

无动物源

≥97%(SDS-PAGE&SEC-HPLC)

神经营养因子，可提高多巴胺能神经元的存活率和形态分化，并增加其对多巴胺的高亲和力摄取。它通过与其核心受体 GFRA1 结合，导致 RET 受体的自身磷酸化和激活。参与神经嵴的发育。

Measured in a cell proliferation assay using SH‑SY5Y human neuroblastoma cells. The ED50 for this effect is 2-12 ng/mL in the presence of Recombinant Human GFRα‑1/GDNF Rα‑1 Fc Chimera. The specific activity of recombinant human GDNF is >5.0 x 10^5 units/mg, which is calibrated against the human GDNF Reference Standard (NIBSC code: 09/266). Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human GFRα‑1/GDNF Rα‑1 Fc Chimera at 1 µg/mL can bind Recombinant Human GDNF with an apparent Kd <1 nM.

重组表达

11.6 kDa (monomer)

No tag

17 kDa, reducing conditions. 33 kDa, non-reducing conditions.

HEK293 Accession #: P39905-1 | HEK293

<1.0 EU/μg

人(Human)

109-211 aa

RGQRGKNRGCVLTAIHLNVTDLGLGYETKEELIFRYCSGSCDAAETTYDKILKNLSRNRRLVSDKVGQACCRPIAFDDDLSFLDDNLVYHILRKHSAKRCGCI

Yes

Yes

-Arg109-Gly-Gln-Arg-Gly-Lys-Asn-Arg-Gly-(Cys)