Recombinant Human TNF-alpha GMP Protein

肿瘤坏死因子重组蛋白 | 重组人 TNF-alpha GMP 蛋白

APC1 protein | Cachectin | Cachetin | DIF | TNF | TNF, monocyte-derived | TNFA | TNF-A | TNFalpha | TNF-alpha | TNF-alphacachectin | TNFATNF, macrophage-derived | TNFG1F | TNLG1F | tumor necrosis factor-alpha | tumor necrosis factor | Tumor necrosis facto

rp168404-GMP-100μg

100μg

无动物源

≥97%(SDS-PAGE&SEC-HPLC)

与 TNFRSF1A/TNFR1 和 TNFRSF1B/TNFBR 结合的细胞因子。它主要由巨噬细胞分泌，能诱导某些肿瘤细胞系的细胞死亡。它是一种强效热原，可通过直接作用或刺激白细胞介素-1分泌引起发热，并与诱导恶病质有关。

Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25-100 pg/mL. The specific activity of recombinant human TNF-α GMP is >4.3 x 10^7 IU/mg, which is calibrated against the human TNF-α WHO International Standard (NIBSC code: 12/154).

重组表达

17 kDa

No tag

17 kDa, under reducing conditions

E. coli Accession #: P01375.1 | E. coli

<0.1 EU/μg

人(Human)

77-233 aa

MVRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL

Yes

Yes

Met-Val77-Arg-Ser-Ser-Ser-Arg-Thr-Pro-Ser Val77-Arg-Ser-Ser-Ser-Arg-Thr-Pro-Ser-Asp