Recombinant Human HA-Ubiquitin Protein

重组人HA-Ubiquitin蛋白 | 重组人 HA-泛素蛋白

RPS27A | UBA52 | UBB ubiquitin B | UBB | UBC | Ubiquitin

rp170435-50μg

50μg

无载体

≥98%(SDS-PAGE)

>98%(SDS-PAGE)

Purity:>95%, by SDS-PAGE visualized with Coomassie® Blue Staining. Description： Ubiquitin is a 76 amino acid (aa) protein that is ubiquitously expressed in all eukaryotic organisms. Ubiquitin is highly conserved with 96% aa sequence identity shared between human and yeast Ubiquitin, and 100% aa sequence identity shared between human and mouse Ubiquitin (1). In mammals, four Ubiquitin genes encode for two Ubiquitin-ribosomal fusion proteins and two poly-Ubiquitin proteins. Cleavage of the Ubiquitin precursors by deubiquitinating enzymes gives rise to identical Ubiquitin monomers each with a predicted molecular weight of 8.6 kDa. Conjugation of Ubiquitin to target proteins involves the formation of an isopeptide bond between the C-terminal glycine residue of Ubiquitin and a lysine residue in the target protein. This process of conjugation, referred to as ubiquitination or ubiquitylation, is a multi-step process that requires three enzymes: a Ubiquitin-activating (E1) enzyme, a Ubiquitin-conjugating (E2) enzyme, and a Ubiquitin ligase (E3). Ubiquitination is classically recognized as a mechanism to target proteins for degradation and as a result, Ubiquitin was originally named ATP-dependent Proteolysis Factor 1 (APF-1). In addition to protein degradation, ubiquitination has been shown to mediate a variety of biological processes such as signal transduction, endocytosis, and post-endocytic sorting.

Testing in progress

重组表达

10.5 kDa

C-His & HA

10.4 kDa, reducing conditions; 10.4 kDa, non-reducing conditions

E. coli Accession #: P0CG47 | E. coli

<1.0 EU/μg

人(Human)

1-76 aa

MHHHHHHYPYDVPDYAQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG

No

Yes