Recombinant Human HGF Protein

重组人HGF蛋白, 重组人肝细胞生长因子. | 重组人HGF蛋白

HGFB | HPTA | HPTAhepatocyte growth factor | lung fibroblast-derived mitogen | Scatter factor | SF | SFhepatopoeitin-A | deafness, autosomal recessive 39 | DFNB39 | EC 3.4.21 | EC 3.4.21.7 | fibroblast-derived tumor cytotoxic factor | F-TCF | hepatocyte g

rp175908-50μg

50μg

无动物源

≥95%(SDS-PAGE)

HGF, also known as scatter factor and hepatopoietin A, is a pleiotropic protein in the plasminogen subfamily of S1 peptidases. It's a multidomain molecule with an N-terminal PAN/APPLE-like domain, four Kringle domains, and a serine proteinase-like domain lacking detectable protease activity. Human HGF is secreted as an inactive single chain propeptide and cleaved to form a bioactive disulfide-linked molecule with alpha and beta chains. Alternate splicing generates human HGF isoforms with variations in the Kringle domains. Human HGF shares high amino acid sequence identity with other mammals. HGF binds to heparan-sulfate proteoglycans and the receptor tyrosine kinase, HGF R/c-MET. HGF-dependent c-MET activation is implicated in cancer development. HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis. It induces the up-regulation of integrin alpha 2 beta 1 in epithelial cells and can alter epithelium morphology. In the thyroid, HGF influences thyrocyte behavior and inhibits iodine uptake. HGF promotes the motility of cardiac stem cells in damaged myocardium. Post-translational: The single-chain precursor undergoes proteolytic processing by TMPRSS13 resulting in an active two-chain form.

1. Determined by the dose-dependent stimulation of the proliferation of monkey 4MBr-5 cells. The ED50 for this effect is 24.77 ng/mL. 2. Immobilized Recombinant Human HGFR/c-MET Protein (rp169642) at 1.0 μg/mL can bind Biotinylated Recombinant Human HGF Protein (rp175908) with the EC50 for this effect is 97.73 ng/mL.

重组表达

80.5 kDa

C-His

30.0-40.0 kDa & 56.0-65.0 kDa & 85.0-95.0 kDa, under reducing condition; 67.0-77.2 kDa, under non-reducing condition

HEK293 Accession #: P14210 | HEK293

<1.0 EU/μg

人(Human)

1-728 aa (D384N, D416N, L622S, H645R, V678I)

MWVTKLLPALLLQHVLLHLLLLPIAIPYAEGQRKRRNTIHEFKKSAKTTLIKIDPALKIKTKKVNTADQCANRCTRNKGLPFTCKAFVFDKARKQCLWFPFNSMSSGVKKEFGHEFDLYENKDYIRNCIIGKGRSYKGTVSITKSGIKCQPWSSMIPHEHSFLPSSYRGKDLQENYCRNPRGEEGGPWCFTSNPEVRYEVCDIPQCSEVECMTCNGESYRGLMDHTESGKICQRWDHQTPHRHKFLPERYPDKGFDDNYCRNPDGQPRPWCYTLDPHTRWEYCAIKTCADNTMNDTDVPLETTECIQGQGEGYRGTVNTIWNGIPCQRWDSQYPHEHDMTPENFKCKDLRENYCRNPDGSESPWCFTTDPNIRVGYCSQIPNCNMSHGQDCYRGNGKNYMGNLSQTRSGLTCSMWNKNMEDLHRHIFWEPDASKLNENYCRNPDDDAHGPWCYTGNPLIPWDYCPISRCEGDTTPTIVNLDHPVISCAKTKQLRVVNGIPTRTNIGWMVSLRYRNKHICGGSLIKESWVLTARQCFPSRDLKDYEAWLGIHDVHGRGDEKCKQVLNVSQLVYGPEGSDLVLMKLARPAVLDDFVSTIDLPNYGCTIPEKTSCSVYGWGYTGSINYDGLLRVAHLYIMGNEKCSQRHRGKVTLNESEICAGAEKIGSGPCEGDYGGPLICEQHKMRMVLGVIVPGRGCAIPNRPGIFVRVAYYAKWIHKIILTYKVPQSHHHHHH

Yes

Yes