Recombinant Human CD3 epsilon Protein

重组人CD3 epsilon蛋白 | 重组人T细胞表面糖蛋白CD3ε链蛋白

CD3 epsilon | CD3e | CD3e antigen | CD3E antigen epsilon polypeptide | CD3e antigen epsilon polypeptide (TiT3 complex) | CD3E antigen, epsilon subunit | CD3e molecule epsilon | CD3e molecule, epsilon (CD3 TCR complex) | CD3e molecule, epsilon (CD3-TCR com

rp175910-10μg

10μg

无动物源

≥90%(SDS-PAGE)

CD3 epsilon (T-Cell Surface Glycoprotein CD3 Epsilon) is a type I transmembrane protein that belongs to the Ig (Immunoglobulin) superfamily. Human CD3 epsilon consists of 104 amino acid (aa) extracellular domain, 26 aa transmembrane domain and 55 aa cytoplasmic domain. With the extracellular domain, human CD3 epsilon shares 44.2% and 49% homology with mouse and rat CD3 epsilon respectively. There are four CD3 proteins: CD3δ, CD3 epsilon, CD3 gamma, and CD3 zeta. All CD3 proteins contain ITAM (Immunoreceptor Tyrosine-based Activation Motifs) in the cytoplasmic tail, which becomes phosphorylated by Src family protein tyrosine kinases LCK and FYN upon TCR engagement. CD3 proteins form heterodimers of CD3δ/CD3 epsilon and CD3 gamma /CD3 epsilon, which bind to TCR and form trimeric TCR alpha /CD3 epsilon /CD3 gamma and TCR beta /CD3 gamma /CD3 epsilon. The resulting heterohexamer further associates with CD3 zeta homodimer and forms TCR/CD3 signaling complex. Similar complex is also formed with TCR gamma and TCRδ. CD3 epsilon plays essential role in adaptive immune response. Mutations in CD3 epsilon lead to SCID (severe combined immunodeficiency). Post-translational: Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8.

Immobilized Recombinant Human CD3 epsilon Protein (rp175910) at 1.0 μg/mL can bind Epcoritamab (anti-CD3&CD20) (Ab175531) with the EC50 of 39.7 ng/mL.

重组表达

12.7 kDa

C-His

13.5-16.0 kDa, under reducing conditions; 15.1 & 26.8 & 42.9 kDa, under non-reducing conditions.

HEK293 Accession #: P07766 | HEK293

<1.0 EU/μg

人(Human)

23-126 aa

MVFTPQILGLMLFWISASRGQDGNEEMGGITQTPYKVSISGTTVILTCPQYPGSEILWQHNDKNIGGDEDDKNIGSDEDHLSLKEFSELEQSGYYVCYPRGSKPEDANFYLYLRARVCENCMEMDHHHHHH​

Yes

Yes