Recombinant Human Estrogen Receptor alpha Protein

重组人Estrogen Receptor alpha蛋白 | 重组人类雌激素受体α蛋白

Estrogen Receptor alpha | ER-alpha | Estradiol receptor | Nuclear receptor subfamily 3 group A member 1 | ER | Era | ESR | ESRA | ESTRR | NR3A | 17*/654 isoform | 7*/819 2 isoform | 7*/822 isoform | 8*/901 isoform | 8*/941 isoform | DKFZp686N23123 | ER al

rp175926-1mg

1mg

无载体

≥90%(SDS-PAGE)

Purity:>90%, by SDS-PAGE visualized with Coomassie® Blue Staining. Description： ER alpha (Estrogen receptor alpha; also Estradiol receptor and NR3A1) is a 65-70 kDa member of the NR3 subfamily, nuclear hormone receptor family of proteins. It is widely expressed, and serves as a strong activator of estrogen-responsive genes. ER alpha is normally quiescent and bound to heat-shock proteins and immunophilins. Following beta -estradiol binding, it becomes activated, either homodimerizes or heterodimerizes with ER beta, and binds to DNA with multiple coactivators. Human ER alpha is 595 amino acids (aa) in length. It contains a DNA binding region (aa 185-250), three NLSs (aa 256-260; 266-271; 299-303), a steroid-binding site (aa 351-543), a dimerization motif (aa 497-518), and an O-GlcNAc attachment around Thr575. Major phosphorylation sites exist at Tyr537, Ser167 and Ser118. Multiple splice forms exist. There is an 80 kDa isoform that shows a substitution (duplication) of aa 412-517 for Asp411, a second isoform with a deletion of aa 255-366, a third isoform with a deletion of aa 152-412, and a fourth isoform that shows a Thr substitution for aa 152-595. Human ER alpha is only 46% aa identical to human ER beta. Over aa 1-116, human ER alpha shares 85% aa identity with mouse ER alpha.

重组表达

21.0 kDa

N-His

24.8kDa and 47.1 kDa, reducing conditions; 24.8kDa and 47.1 kDa, non-reducing conditions

E. coli Accession #: P03372 | E. coli

<1.0 EU/μg

人(Human)

1-185 aa

MHHHHHHMTMTLHTKASGMALLHQIQGNELEPLNRPQLKIPLERPLGEVYLDSSKPAVYNYPEGAAYEFNAAAAANAQVYGQTGLPYGPGSEAAAFGSNGLGGFPPLNSVSPSPLMLLHPPPQLSPFLQPHGQQVPYYLENEPSGYTVREAGPPAFYRPNSDNRRQGGRERLASTNDKGSMAMESAKETRYC

No

Yes