Recombinant Human Isocitrate Dehydrogenase 1/IDH1 Protein

重组人Isocitrate Dehydrogenase 1/IDH1蛋白 | 重组人异柠檬酸脱氢酶 (NADP (+) ) 1蛋白

Cytosolic NADP-isocitrate dehydrogenase | EC 1.1.1.42 | IDCD | IDH | IDH1 | IDP | IDPC | isocitrate dehydrogenase [NADP] cytoplasmic | isocitrate dehydrogenase 1 (NADP+), soluble | Isocitrate Dehydrogenase 1 | NADP(+)-specific ICDH | NADP-dependent isocit

rp179799-10μg

10μg

无载体

≥95%(SDS-PAGE)

Isocitrate Dehydrogenase 1 (IDH1) catalyzes the oxidative decarboxylation of isocitrate to alpha ‑ketoglutarate. There are two subclasses in the IDH family, one of them utilizing NADP+ as the electron acceptor and the other using NAD+. The protein encoded by this gene is the NADP+-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. In peroxisomes, IDH1 generates the NADPH required for intraperoxisomal reduction reactions. Mutations of Arg132 of human IDH1 result in a reduced ability of the enzyme to convert isocitrate to alpha ‑ketoglutarate, but the enzyme acquires the ability to generate 2-hydroxyglutarate (2HG) from alpha ‑ketoglutarate. Elevated levels of the metabolite 2HG are associated with a high risk of malignant brain tumors. Arg132 mutations of IDH1 are common in high‑grade gliomas, but not in other types of tumors. Post-translational: Acetylation at Lys-374 dramatically reduces catalytic activity.

1、Measured by the ability to oxidatively decarboxylate isocitrate to 2-oxoglutarate. The specific activity is >10,000 pmol/min/μg, as measured under the described conditions. 2、Immobilized Recombinant Human Isocitrate Dehydrogenase 1/IDH1 Protein (rp179799) at 3.0 μg/mL IDH1 Mouse mAb (Ab109324) with the EC50 of 11.16 ng/mL.

重组表达

47.5 kDa

C-His

44.2 kDa, under reducing conditions; 43.6 kDa, under non-reducing conditions.

E. coli Accession #: O75874-1 | E. coli

<1.0 EU/μg

人(Human)

1-414 aa

MSKKISGGSVVEMQGDEMTRIIWELIKEKLIFPYVELDLHSYDLGIENRDATNDQVTKDAAEAIKKHNVGVKCATITPDEKRVEEFKLKQMWKSPNGTIRNILGGTVFREAIICKNIPRLVSGWVKPIIIGRHAYGDQYRATDFVVPGPGKVEITYTPSDGTQKVTYLVHNFEEGGGVAMGMYNQDKSIEDFAHSSFQMALSKGWPLYLSTKNTILKKYDGRFKDIFQEIYDKQYKSQFEAQKIWYEHRLIDDMVAQAMKSEGGFIWACKNYDGDVQSDSVAQGYGSLGMMTSVLVCPDGKTVEAEAAHGTVTRHYRMYQKGQETSTNPIASIFAWTRGLAHRAKLDNNKELAFFANALEEVSIETIEAGFMTKDLAACIKGLPNVQRSDYLNTFEFMDKLGENLKIKLAQAKLHHHHHH

No

Yes