Recombinant Human VISTA/B7-H5 Protein

重组人VISTA/B7-H5/PD-1H蛋白 | 重组人 VISTA/B7-H5 蛋白

B7H5 | C10orf54 | chromosome 10 open reading frame 54 | DD1alpha | GI24 | GI24_HUMAN | PD-1H | PDCD1 homolog | Platelet receptor Gi24 | PP2135 | sisp 1 | SISP1 | stress induced secreted protein 1 | B7 H5 | UNQ730/PRO1412 | V domain Ig suppressor of T cell

rp181308-10μg

10μg

无动物源

≥95%(SDS-PAGE)

Protein Function: Platelet receptor Gi24, also known as Dies1, VISTA, SISP1 and B7-H5, is a 55-65 kDa transmembrane glycoprotein with homology to B7-like immune costimulatory molecules. Mature human Gi24 contains a 162 amino acid (aa) extracellular domain (ECD) with one V-type Ig-like domain, a 21 aa transmembrane segment, and a 96 aa cytoplasmic domain. Within the ECD, human Gi24 shares 70% and 67% aa sequence identity with mouse and rat Gi24, respectively. The 30 kDa ECD can be shed by MT1-MMP, with a 25-30 kDa fragment remaining in the membrane. Gi24 promotes both MT1-MMP expression and the MT1-MMP mediated activation of MMP-2. Gi24 supports the differentiation of embryonic stem cells (ESC) and enhances BMP-4 induced signaling in ESC, but is also down-regulated following BMP-4 exposure. It binds to BMP-4 directly, and also associates with the type I BMP receptor Activin RIB/ALK-4. Gi24 is expressed on the surface of naïve CD4+ T cells, regulatory T cells, and adipocytes. It is up-regulated in vivo on activated monocytes and dendritic cells. Gi24 inhibits CD4+ and CD8+ T cell proliferation, and their production of IL-2 and IFN-gamma. Its expression on tumor cells attenuates the anti-tumor immune response and enables more rapid tumor progression. In contrast, Gi24 limits disease progression in the autoimmune disease model EAE. Gi24 also acts as a co-inhibitory receptor on alloreactive T cells to regulate T cell tolerance.

Immobilized Recombinant Human VISTA/B7-H5/PD-1H Protein (rp181308) at 1.0 μg/mL can bind Onvatilimab (anti-VISTA) (Ab170685) with the EC50 of 48.93 ng/mL.

重组表达

22.2 kDa

C-His

33.0-46.3 kDa, under reducing conditions; 31.3-43.6 kDa, under non-reducing conditions

HEK293 Accession #: Q9H7M9 | HEK293

<1.0 EU/μg

人(Human)

1-194 aa

MGVPTALEAGSWRWGSLLFALFLAASLGPVAAFKVATPYSLYVCPEGQNVTLTCRLLGPVDKGHDVTFYKTWYRSSRGEVQTCSERRPIRNLTFQDLHLHHGGHQAANTSHDLAQRHGLESASDHHGNFSITMRNLTLLDSGLYCCLVVEIRHHHSEHRVHGAMELQVQTGKDAPSNCVVYPSSSQESENITAAHHHHHH

Yes

Yes