Recombinant Human 5'-nucleotidase/CD73 Protein

重组人5'-nucleotidase/CD73蛋白 | 重组人跨膜糖蛋白

5' nucleotidase (CD73) | 5'-NT | 5-NT | 5'-Nucleotidase | 5'-nucleotidase, ecto (CD73) | CD73 antigen | CD73 | E5NT | EC 3.1.3.5 | ecto-5'-nucleotidase | eN | eNT | NT | NT55'-nucleotidase | NT5E | NTE | Purine 5-Prime-Nucleotidase

rp181611-10μg

10μg

无动物源

≥95%(SDS-PAGE)

5'-nucleotidase, also known as NT5E, NTE, and CD73, is a cell membrane protein that belongs to the 5'-nucleotidase family. CD73 is a glycosylphosphatidylinositol (GPI) anchored purine salvage enzyme expressed on the surface of human T and B lymphocytes. CD73 catalyzes the conversion of purine and pyrimidine ribo- and deoxyribonucleoside monophosphates to the corresponding nucleosides. CD73 serves as a costimulatory molecule in activating T cells. CD73 generated adenosine functions in cell signaling in many physiologic systems, including intestinal epithelium, ischemic myocardium, and cholinergic synapses. CD73 might mediate lymphocyte-stromal cell interactions or condition the local microenvironment to facilitate lymphocyte development and/or function. In CD73-depleted cells, surface levels of the leukocyte adhesion molecules ICAM-1, VCAM-1, and E-selectin increase. CD73 produces extracellular adenosine, which then acts on G protein-coupled purinergic receptors to induce cellular responses. CD73 has also been reported to regulate the expression of pro-inflammatory molecules in mouse endothelium.

Immobilized Recombinant Human 5'-nucleotidase/CD73 Protein (rp181611) at 1.0 μg/mL can bind Mupadolimab (anti-CD73) (Ab170487) with the EC50 of 13.1 ng/mL. Immobilized Recombinant Human 5'-nucleotidase/CD73 Protein (rp181611) at 1.0 μg/mL can bind Oleclumab (anti-CD73) (Ab170578) with the EC50 of 2.1 ng/mL.

重组表达

58.6 kDa

C-His

61.1 kDa, under reducing conditions; 61.7 kDa, under non-reducing conditions.

HEK293 Accession #: P21589-1 | HEK293

<1.0 EU/μg

人(Human)

1-547 aa

MCPRAARAPATLLLALGAVLWPAAGAWELTILHTNDVHSRLEQTSEDSSKCVNASRCMGGVARLFTKVQQIRRAEPNVLLLDAGDQYQGTIWFTVYKGAEVAHFMNALRYDAMALGNHEFDNGVEGLIEPLLKEAKFPILSANIKAKGPLASQISGLYLPYKVLPVGDEVVGIVGYTSKETPFLSNPGTNLVFEDEITALQPEVDKLKTLNVNKIIALGHSGFEMDKLIAQKVRGVDVVVGGHSNTFLYTGNPPSKEVPAGKYPFIVTSDDGRKVPVVQAYAFGKYLGYLKIEFDERGNVISSHGNPILLNSSIPEDPSIKADINKWRIKLDNYSTQELGKTIVYLDGSSQSCRFRECNMGNLICDAMINNNLRHTDEMFWNHVSMCILNGGGIRSPIDERNNGTITWENLAAVLPFGGTFDLVQLKGSTLKKAFEHSVHRYGQSTGEFLQVGGIHVVYDLSRKPGDRVVKLDVLCTKCRVPSYDPLKMDEVYKVILPNFLANGGDGFQMIKDELLRHDSGDQDINVVSTYISKMKVIYPAVEGRIKHHHHHH

Yes

Yes