Recombinant Human Alkaline Phosphatase/ALPL Protein

重组人Alkaline Phosphatase/ALPL蛋白 | 重组人非组织特异性碱性磷酸酶蛋白

Akp2 | Alkaline phosphatase liver/bone/kidney isozyme | alkaline phosphatase, liver/bone/kidney | alkaline phosphatase, tissue-nonspecific isozyme | alkaline phosphomonoesterase | ALPL | APTNAP | AP-TNAP | EC 3.1.3.1 | FLJ40094 | FLJ93059 | glycerophospha

rp183653-10μg

10μg

无动物源

≥95%(SDS-PAGE)

Alkaline phosphatase (ALPL) is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. The process of removing the phosphate group is called dephosphorylation. As the name suggests, alkaline phosphatases are most effective in an alkaline environment. It is sometimes used synonymously as basic phosphatase. Alkaline phosphatases (APs) are ubiquitous in many species, from bacteria to human. Four genes encode AP isoenzymes in humans and rodents. Three AP genes are expressed in a tissue-specific manner (i.e., placental, embryonic, and intestinal AP isoenzymes). Expression of the fourth AP gene is nonspecific to a single tissue and is especially abundant in bone, liver, and kidney. This isoenzyme is also called tissue-nonspecific alkaline phosphatase (TNAP). The enzyme tissue non-specific alkaline phosphatase (TNAP) belongs to the ectophosphatase family. TNAP is present in large amounts in bone in which it plays a role in mineralization. Post-translational: N-glycosylated.

Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl phosphate (4-MUP) (M103814). The specific activity is >30,000 pmol/min/µg.

重组表达

54.2 kDa

C-His

59.1-72.1 kDa, under reducing conditions; 57.7-70.8 kDa, under non-reducing conditions.

HEK293 Accession #: P05186 | HEK293

<1.0 EU/μg

人(Human)

1-502

MISPFLVLAIGTCLTNSLVPEKEKDPKYWRDQAQETLKYALELQKLNTNVAKNVIMFLGDGMGVSTVTAARILKGQLHHNPGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTATAYLCGVKANEGTVGVSAATERSRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHATPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIRDIDVIMGGGRKYMYPKNKTDVEYESDEKARGTRLDGLDLVDTWKSFKPRYKHSHFIWNRTELLTLDPHNVDYLLGLFEPGDMQYELNRNNVTDPSLSEMVVVAIQILRKNPKGFFLLVEGGRIDHGHHEGKAKQALHEAVEMDRAIGQAGSLTSSEDTLTVVTADHSHVFTFGGYTPRGNSIFGLAPMLSDTDKKPFTAILYGNGPGYKVVGGERENVSMVDYAHNNYQAQSAVPLRHETHGGEDVAVFSKGPMAHLLHGVHEQNYVPHVMAYAACIGANLGHCAPASSHHHHHH

Yes

Yes