Recombinant Human CCL3L1/LD78 beta Protein

重组人CCL3L1/LD78 beta蛋白 | 重组人CCL3样蛋白1

C-C motif chemokine 3-like 1 | CCL3L1 | chemokine (C-C motif) ligand 3-like 3 | chemokine (C-C motif) ligand 3-like | centromeric | D17S1718 | G0/G1 switch regulatory protein 19-2 | G0S19-2 | LD78 beta | LD78 | LD78BETA | LD78-beta(1-70) | MGC12815 | MIP1

rp183664-10μg

10μg

无载体

≥95%(SDS-PAGE)

The macrophage inflammatory protein-1 alpha (MIP-1 alpha )/CCL3 and-1 beta (MIP-1 beta )/CCL4 are highly homologous CC family chemokines that were originally co-purified from medium conditioned by an LPS-stimulated murine macrophage cell line. Mouse MIP-1 alpha and MIP-1 beta are encoded by their respective single-copy genes. In human, additional non-allelic MIP-1 alpha and MIP-1 beta genes also exist. The two human MIP-1 alpha genes arise by duplication/mutation. They code for MIP-1 alpha isoforms CCL3/LD78 alpha and CCL3L1/LD78 beta, which share 94% amino acid (aa) sequence homology. Whereas the human CCL3/LD78 alpha is a single-copy gene, the human CCL3L1/LD78 beta gene copy number varies within the population. Human CCL3L1/LD78 beta cDNA encodes a 93 aa residue precursor with a 23 aa residue signal peptide that is cleaved to generate a 70 aa mature protein. Human CCL3L1/LD78 beta binds and signals through chemokine receptors CCR1 and CCR5. When compared to CCL3/LD78 alpha, CCL3L1/LD78 beta has higher binding affinity to CCR5, which also functions as a coreceptor for HIV-1 entry. The copy number of CCL3L1 is one of several genetic determinants of HIV-1 susceptibility. Post-translational: The N-terminal processed forms LD78-beta(3-70) and LD78-beta(5-70) are produced by proteolytic cleavage after secretion from peripheral blood monocytes. The cleavage to yield LD78-beta(3-70) is probably achieved by DPP4.

Determined by its ability to chemoattract human monocytes using a concentration range of 0 - 5.0 ng/mL.

重组表达

22.1 kDa

No tag

10.0 & 16.8 kDa, under reducing conditions; 10.8 & 19.0 kDa, under non-reducing conditions.

E. coli Accession #: P16619 | E. coli

<1.0 EU/μg

人(Human)

24-93 aa

GAPLAADTPTACCFSYTSRQIPQNFIADYFETSSQCSKPSVIFLTKRGRQVCADPSEEWVQKYVSDLELSA

No

Yes