Recombinant Human SMAC/Diablo Protein

重组人SMAC/Diablo蛋白 | 重组人线粒体促凋亡蛋白

0610041G12Rik | Diablo | diablo, IAP-binding mitochondrial protein | Direct IAP-binding protein with low pI | mitochondrial Smac protein | Second mitochondria-derived activator of caspase | SMAC | SMACmitochondrial

rp183671-10μg

10μg

无载体

≥95%(SDS-PAGE)

Apoptosis is an essential processes required for normal development and homeostasis of all metazoan organisms. Second Mitochondria-Derived Activator of Caspases (Smac) or Direct IAP Binding Protein with low isoelectric point, pI (Diablo) is a proapoptogenic mitochondrial protein that is released to the cytosol in response to diverse apoptotic stimuli, including commonly used chemotherapeutic drugs. The current knowlege about structure and function of Smac/Diablo during programmed cell death, both in mitochondrial and receptor pathways are presented. It has been shown that Diablo mainly interacts with IAPs in the cytochrome c/Apaf-1/caspase-9 pathway, and promotes apoptosis. Diablo is released from the mitochondria into the cytosol occurring downstream of cytochrome c release in response to apoptotic stimuli such as irradiation, DNA damage or cytotoxic drugs. In the cytosol, Smac/Diablo interacts and antagonizes inhibitors of apoptosis proteins (IAPs), thus allowing the activation of caspases and apoptosis. This activity has prompted the synthesis of peptidomimetics that could potentially be used in cancer therapy. The role of Smac/DIABLO in colorectal carcinogenesis is ill defined. Data continues to accumulate to suggest that decreased levels of Smac/DIABLO may be important in chemoradiation-resistance to apoptosis in advanced colon cancer. Post-translational: Ubiquitinated by BIRC7/livin. Ubiquitinated by BIRC6. The precursor form is proteolytically cleaved by mitochondrial processing peptidase MPP to remove the transit peptide and produce an intermediate form. This is then processed by PARL to produce the mature cleaved form which is released from mitochondria into the cytosol in apoptotic cells.

Immobilized Recombinant Human SMAC/Diablo Protein (rp183671) at 1.0 μg/mL can bind Recombinant Smac/Diablo Antibody (Ab128141) with the EC50 of 132.8 ng/mL.

重组表达

21.7 kDa

C-His

20.6 kDa, under reducing conditions; 20.6 kDa, under non-reducing conditions.

E. coli Accession #: Q9NR28 | E. coli

<1.0 EU/μg

人(Human)

56-239 aa

MAVPIAQKSEPHSLSSEALMRRAVSLVTDSTSTFLSQTTYALIEAITEYTKAVYTLTSLYRQYTSLLGKMNSEEEDEVWQVIIGARAEMTSKHQEYLKLETTWMTAVGLSEMAAEAAYQTGADQASITARNHIQLVKLQVEEVHQLSRKAETKLAEAQIEELRQKTQEEGEERAESEQEAYLREDHHHHHH

No

Yes