Recombinant Human/Mouse/Rat UBE2M/Ubc12 Protein

重组人/小鼠/大鼠UBE2M/Ubc12蛋白 | 重组人/小鼠/大鼠参与 NEDD8 偶联的 Ubc12 酶蛋白

EC 6.3.2 | EC 6.3.2.- | hUbc12 | NEDD8 carrier protein | NEDD8 protein ligase | UBC12 | UBC12NEDD8-conjugating enzyme Ubc12 | UbcH12 | UBC-RS2 | UBE2M | ubiquitin carrier protein M | Ubiquitin-conjugating enzyme E2 M | ubiquitin-conjugating enzyme E2M (ho

rp183713-10μg

10μg

无载体

≥95%(SDS-PAGE)

Ubiquitin-conjugating Enzyme E2M (UBE2M), also known as Ubiquitin-conjugating Enzyme 12 (Ubc12), is a ubiquitously expressed protein with a predicted molecular weight of 20 kDa. UBE2M/Ubc12 is highly conserved, and the human ortholog shares 100% amino acid identity with mouse and rat UBE2M. UBE2M/Ubc12 mediates conjugation of the Ubiquitin-like protein Neural Precursor Cell Expressed, Developmentally Down-regulated 8 (NEDD8) to substrate proteins. Neddylation requires the coordinated activities of UBE2M/Ubc12 and the APPBPI/UBA3 heterodimer, a NEDD8-activating (E1) enzyme. The NEDD8 pathway is essential for the viability of many organisms and plays important roles in morphogenesis and cell cycle progression. UBE2M/Ubc12-dependent neddylation modulates the levels of p27/Kip1, beta-Catenin, Cdc53/Cul1, and other members of the Cullin family. Post-translational: The acetylation of Met-1 increases affinity for DCUN1D1 by about 2 orders of magnitude and is crucial for NEDD8 transfer to cullins.

Immobilized Recombinant Human/Mouse/Rat UBE2M/Ubc12 Protein (rp183713) at 1.0 μg/mL can bind UBE2M/UBC12 Mouse mAb (Ab133254) with the EC50 of 25.4 ng/mL.

重组表达

20.8 kDa

No tag

19.2 kDa, under reducing conditions; 19.2 kDa, under non-reducing conditions.

E. coli Accession #: P61081 | E. coli

<1.0 EU/μg

人(Human)

2-183 aa

IKLFSLKQQKKEEESAGGTKGSSKKASAAQLRIQKDINELNLPKTCDISFSDPDDLLNFKLVICPDEGFYKSGKFVFSFKVGQGYPHDPPKVKCETMVYHPNIDLEGNVCLNILREDWKPVLTINSIIYGLQYLFLEPNPEDPLNKEAAEVLQNNRRLFEQNVQRSMRGGYIGSTYFERCLK

No

Yes