Recombinant Human SNAIL Protein

重组人SNAIL蛋白 | 重组人锌指蛋白SNAI1

dJ710H13.1 | Protein sna | Protein snail homolog | Protein snail homolog 1 | SLUGH2 | SNA | Sna protein | SNAH | SNAI | snai1 | SNAI1_HUMAN | SNAIL | Snail 1 homolog | Snail 1 zinc finger protein | Snail family transcriptional repressor 1 | Snail homolog

rp184134-10μg

10μg

无载体

≥95%(SDS-PAGE)

Involved in induction of the epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, survival and cell migration. Binds to 3 E-boxes of the E-cadherin/CDH1 gene promoter and to the promoters of CLDN7 and KRT8 and, in association with histone demethylase KDM1A which it recruits to the promoters, causes a decrease in dimethylated H3K4 levels and represses transcription. Associates with EGR1 and SP1 to mediate tetradecanoyl phorbol acetate (TPA)-induced up-regulation of CDKN2B, possibly by binding to the CDKN2B promoter region 5'-TCACA-3. In addition, may also activate the CDKN2B promoter by itself. Post-translational: Phosphorylated by GSK3B (PubMed:15448698, PubMed:15647282, PubMed:20305697, PubMed:25827072, PubMed:29059170). Once phosphorylated, it becomes a target for ubiquitination by BTRC, the ECS(SPSB3) or the SCF(FBXO11) complexes (PubMed:15647282, PubMed:29059170). Phosphorylation by CSNK1E, probably at Ser-104, provides the priming site for the subsequent phosphorylation by GSK3B, probably at Ser-100 and Ser-96 (PubMed:19923321, PubMed:20305697). Phosphorylation by PAK1 may modulate its transcriptional activity by promoting increased accumulation in the nucleus (PubMed:15833848). Phosphorylation at Ser-11 and Ser-92 positively regulates its functions in induction of EMT and cell survival, respectively (PubMed:19923321). Phosphorylation by LATS2, upon mitotic stress, oncogenic stress or Hippo pathway activation, occurs in the nucleus and promotes nuclear retention and stabilization of total cellular protein level (PubMed:21952048, PubMed:24157836). Ubiquitinated on Lys-98, Lys-137 and Lys-146 by FBXL14 and BTRC leading to degradation (PubMed:19955572). BTRC-triggered ubiquitination requires previous GSK3B-mediated SNAI1 phosphorylation (PubMed:19955572). Ubiquitinated by the SCF(FBXO11) complex; ubiquitination requires previous GSK3B-mediated SNAI1 phosphorylation (PubMed:25827072). Ubiquitinated by the ECS(SPSB3) complex; ubiquitination requires previous GSK3B-mediated SNAI1 phosphorylation (PubMed:29059170). Ubiquitination induced upon interaction with NOTCH1 or TP53/p53 is mediated by MDM2 (PubMed:20385133). Ubiquitinated in a FBXL5-dependent manner; preventing interaction with DNA and promoting its degradation (PubMed:24157836). Deubiquitinated by USP37; leading to stabilization (PubMed:31911859). O-GlcNAcylation at Ser-112 is enhanced in hyperglycaemic conditions, it opposes phosphorylation by GSK3B, and stabilizes the protein. ADP-ribosylation by PARP1 increases protein half-life and may be involved in TGFB-induced SNAI1 up-regulation.

重组表达

29.9 kDa

N-His

30.5 kDa, under reducing conditions; 29.9 & 62.1 & 113.6 kDa, under non-reducing conditions.

E. coli Accession #: O95863 | E. coli

<1.0 EU/μg

人(Human)

2-264 aa

MHHHHHHPRSFLVRKPSDPNRKPNYSELQDSNPEFTFQQPYDQAHLLAAIPPPEILNPTASLPMLIWDSVLAPQAQPIAWASLRLQESPRVAELTSLSDEDSGKGSQPPSPPSPAPSSFSSTSVSSLEAEAYAAFPGLGQVPKQLAQLSEAKDLQARKAFNCKYCNKEYLSLGALKMHIRSHTLPCVCGTCGKAFSRPWLLQGHVRTHTGEKPFSCPHCSRAFADRSNLRAHLQTHSDVKKYQCQACARTFSRMSLLHKHQESGCSGCPR

No

Yes