Recombinant Human CDK2 Protein

重组人CDK2蛋白 | 重组人细胞周期蛋白依赖性激酶2

Cdc2 related protein kinase | cdc2-related protein kinase | CDC28 | CDC2A | Cdk 2 | CDK1 | CDK2 | CDK2_HUMAN | CDKN2 | Cell devision kinase 2 | Cell division protein kinase 2 | Cyclin dependent kinase 2 | cyclin dependent kinase 2-alpha | Cyclin-dependent

rp188224-10μg

10μg

无载体

≥95%(SDS-PAGE)

Cyclin-dependent kinase 2 (CDK2) belongs the Ser/Thr protein kinase family. CDK2 is highly parallel to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. CDK2 is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is limited to the G1-S phase, and is vital for cell cycle G1/S phase transition. The CDK2 protein associates with and is regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). CDK2 activity is also regulated by protein phosphorylation. Post-translational: Phosphorylated at Thr-160 by CDK7 in a CAK complex (PubMed:28666995). Phosphorylation at Thr-160 promotes kinase activity, whereas phosphorylation at Tyr-15 by WEE1 reduces slightly kinase activity. Phosphorylated on Thr-14 and Tyr-15 during S and G2 phases before being dephosphorylated by CDC25A. Nitrosylated after treatment with nitric oxide (DETA-NO).

Immobilized Recombinant Human CDK2 Protein (rp188224) at 1.0 μg/mL can bind CDK2 Mouse mAb (Ab095749) with the EC50 of 62.4 ng/mL.

重组表达

34.8 kDa

N-His

33.2 kDa, under reducing conditions; 33.2 kDa, under non-reducing conditions.

E. coli Accession #: P24941 | E. coli

<1.0 EU/μg

人(Human)

2-298 aa

MHHHHHHENFQKVEKIGEGTYGVVYKARNKLTGEVVALKKIRLDTETEGVPSTAIREISLLKELNHPNIVKLLDVIHTENKLYLVFEFLHQDLKKFMDASALTGIPLPLIKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINTEGAIKLADFGLARAFGVPVRTYTHEVVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVTRRALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWARQDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKPVPHLRL

No

Yes