Recombinant Human OTUB2 Protein

重组人OTUB2蛋白 | 重组人泛素硫酯酶OTUB2蛋白

Ubiquitin thioesterase OTUB2 | Deubiquitinating enzyme OTUB2 | OTU domain-containing ubiquitin aldehyde-binding protein 2 | Otubain-2, Ubiquitin-specific-processing protease OTUB2 | OTUB2 | C14orf137 | OTB2 | OTU2.

rp188262-10μg

10μg

无载体

≥95%(SDS-PAGE)

Otubain 2 (OTUB2) is a member of DUBs that belong to the ovarian tumour (OTU) superfamily of proteins which consists of a five-stranded β-sheet sandwiched in between a small helical amino-terminal region consisting of α1 and α2, and a large helical region comprised of α3-α10. Like other DUBs, otubain 2 (OTUB2) cleaves proteins precisely at the ubiquitin-protein bond so that ubiquitylation process can be reversed and regulated. Otubain 2 (OTUB2)'s active-site cleft is sterically occluded by a novel loop conformation resulting in an oxyanion hole, which consists uniquely of backbone amides. Furthermore, the residues that orient and stabilize the active-site histidine of otubain 2 (OTUB2) are different from other cysteine proteases. This reorganization of the active-site topology provides a possible explanation for the low turnover and substrate specificity of the otubains.

Immobilized Recombinant Human OTUB2 Protein (rp188262) at 1.0 μg/mL can bind OTUB2 Antibody (Ab119481) with the EC50 of 301.8 ng/mL.

重组表达

28.0 kDa

N-His

28.1 kDa, under reducing conditions; 28.1 kDa, under non-reducing conditions.

E. coli Accession #: Q96DC9 | E. coli

<1.0 EU/μg

人(Human)

2-234 aa

MHHHHHHSETSFNLISEKCDILSILRDHPENRIYRRKIEELSKRFTAIRKTKGDGNCFYRALGYSYLESLLGKSREIFKFKERVLQTPNDLLAAGFEEHKFRNFFNAFYSVVELVEKDGSVSSLLKVFNDQSASDHIVQFLRLLTSAFIRNRADFFRHFIDEEMDIKDFCTHEVEPMATECDHIQITALSQALSIALQVEYVDEMDTALNHHVFPEAATPSVYLLYKTSHYNILYAADKH

No

Yes