Recombinant Human Dihydrolipoamide Dehydrogenase/DLD Protein

重组人Dihydrolipoamide Dehydrogenase/DLD蛋白 | 重组人二氢硫辛酰胺脱氢酶蛋白

2-oxo-glutarate complex | branched chain keto acid dehydrogenase complex | Diaphorase | dihydrolipoamide dehydrogenase (E3 component of pyruvate dehydrogenase complex | Dihydrolipoamide Dehydrogenase | dihydrolipoamide dehydrogenasemitochondrial | DLD | D

rp188307-10μg

10μg

无载体

≥95%(SDS-PAGE)

Dihydrolipoamide dehydrogenase (DLD), also known as LADH, is an NAD-dependent oxidoreductase in the mitochondrial matrix. DLD serves as the E3 subunit of four mitochondrial enzyme complexes: pyruvate dehydrogenase, alpha-ketoglutarate dehydrogenase, branched chain alpha-ketotacid dehydrogenase, and the glycine cleavage system. It is active as a 120 kDa dimer that catalyzes oxidation within these enzyme complexes. Several mutations of human DLD have been described, some of which contribute to the loss of respiratory function during oxidative stress. DLD mutations located at the interface between dimer subunits can impair dimer formation. The involvement of DLD in the regulation of lipid peroxidation and lactate metabolism is important for mouse hippocampal neuroblast proliferation and hamster sperm capacitation, respectively. DLD polymorphisms in insects can increase their resistance to the pesticide phosphine gas, while they can increase the sensitivity to arsenite in the nematode C. elegans. Mature human DLD shares 95-96% amino acid (aa) sequence identity with hamster, mouse, and rat DLD. Alternative splicing generates additional human DLD isoforms that lack the N-terminal 99 aa or carry a deletion of aa 147-194. Post-translational: Tyrosine phosphorylated.

Testing in progress

重组表达

51.1 kDa

N-His

52.0 kDa, under reducing conditions; 52.0 kDa, under non-reducing conditions.

E. coli Accession #: P09622 | E. coli

<1.0 EU/μg

人(Human)

36-509 aa

MHHHHHHADQPIDADVTVIGSGPGGYVAAIKAAQLGFKTVCIEKNETLGGTCLNVGCIPSKALLNNSHYYHMAHGKDFASRGIEMSEVRLNLDKMMEQKSTAVKALTGGIAHLFKQNKVVHVNGYGKITGKNQVTATKADGGTQVIDTKNILIATGSEVTPFPGITIDEDTIVSSTGALSLKKVPEKMVVIGAGVIGVELGSVWQRLGADVTAVEFLGHVGGVGIDMEISKNFQRILQKQGFKFKLNTKVTGATKKSDGKIDVSIEAASGGKAEVITCDVLLVCIGRRPFTKNLGLEELGIELDPRGRIPVNTRFQTKIPNIYAIGDVVAGPMLAHKAEDEGIICVEGMAGGAVHIDYNCVPSVIYTHPEVAWVGKSEEQLKEEGIEYKVGKFPFAANSRAKTNADTDGMVKILGQKSTDRVLGAHILGPGAGEMVNEAALALEYGASCEDIARVCHAHPTLSEAFREANLAASFGKSINF​

No

Yes