Recombinant Human KRAS (G12S) Protein

重组人KRAS (G12S)蛋白 | 重组人Kirsten大鼠肉瘤病毒癌基因同源物(G12S)蛋白

C-K-RAS Protein | c-Ki-ras Protein | c-Ki-ras2 Protein | CFC2 Protein | K-Ras Protein | K-Ras 2 Protein | K-RAS2A Protein | K-RAS2B Protein | K-RAS4A Protein | K-RAS4B Protein | KI-RAS Protein | KRAS1 Protein | KRAS2 Protein | NS Protein | NS3 Protein | O

rp192049-10μg

10μg

无载体

≥95%(SDS-PAGE)

K-Ras belongs to the small GTPase superfamily, Ras family. Like other members of the Ras family, K-Ras is a GTPase and is an early player in many signal transduction pathways. It is usually tethered to cell membranes because of the presence of an isoprenyl group on its C-terminus. K-Ras functions as a molecular on/off switch. Once it is turned on it recruits and activates proteins necessary for the propagation of growth factor and other receptors' signal, such as c-Raf and PI 3-kinase. It binds to GTP in the active state and possesses an intrinsic enzymatic activity that cleaves the terminal phosphate of the nucleotide converting it to GDP. Upon conversion of GTP to GDP, K-Ras is turned off. The rate of conversion is usually slow but can be sped up dramatically by an accessory protein of the GTPase activating protein class, for example, RasGAP. In turn, K-Ras can bind to proteins of the Guanine Nucleotide Exchange Factor class, for example, SOS1, which forces the release of bound nucleotide. Subsequently, K-Ras binds GTP present in the cytosol and the GEF is released from ras-GTP. Besides essential function in normal tissue signaling, the mutation of a K-Ras gene is an essential step in the development of many cancers. Several germline K-Ras mutations are associated with Noonan syndrome and Cardio-Facio-Cutaneous syndrome. Somatic K-Ras mutations are found at high rates in Leukemias, colon cancer, pancreatic cancer, and lung cancer. Post-translational: Acetylation at Lys-104 prevents interaction with guanine nucleotide exchange factors (GEFs). Palmitoylated at Lys-182, Lys-184 and Lys-185. Palmitoylated at Lys-182, Lys-184 and Lys-185. Palmitoylation on lysine residues is promoted by palmitoylation at Cys-180. Lysine-depalmitoylation by SIRT2 promotes its localization to endomembranes in endocytic pathways. Ubiquitinated by the BCR(LZTR1) E3 ubiquitin ligase complex at Lys-170 in a non-degradative manner, leading to inhibit Ras signaling by decreasing Ras association with membranes. (Microbial infection) Glucosylated at Thr-35 by P.sordellii toxin TcsL.

The specific activity of KRAS was determined to be >3.0 nmol/min/mg in a GTPase-Glo assay using GTP solution substrate.

重组表达

21.9 kDa

N-His

22.1 kDa, under reducing conditions; 22.8 kDa, under non-reducing conditions

E. coli Accession #: P01116-2 | E. coli

<1.0 EU/μg

人(Human)

2-185 aa (G12S)

No

Yes