Recombinant Human S100A9 Protein

重组人S100A9蛋白 | 重组人S100钙结合蛋白A9

CAGBMigration inhibitory factor-related protein 14 | Calgranulin B | calgranulin-B | Calprotectin L1H subunit | CFAGMRP-14,60B8AG | CGLB | L1AG | LIAG | MAC387 | MIF | MRP-14 | MRP14Leukocyte L1 complex heavy chain | NIF | P14 | protein S100-A9 | S100 cal

rp209720-10μg

10μg

无载体

≥95%(SDS-PAGE)

S100A9, also known as MRP14 and Calgranulin B, is an approximately 14 kDa pro-inflammatory protein in the S100 family of calcium-binding proteins. It is up-regulated in neutrophils and monocytes at sites of inflammation (e.g., psoriasis, rheumatoid arthritis, cardiac ischemia) and is present at elevated concentrations in rheumatoid arthritis synovial fluid. Human S100A9 contains two EF-hand calcium-binding motifs and shares 57% and 62% amino acid sequence identity with mouse and rat S100A9, respectively. S100A9 is a noncovalent homodimer that can also noncovalently heterodimerize with S100A8; in the presence of calcium and zinc, the homodimer and heterodimers will form tetramers. Human S100A9 can be phosphorylated at Thr113 near the C-terminus, a residue that is not conserved in the mouse protein. Human S100A9 can also be modified by S-glutathionylation and oxidation. The S100A8/A9 heterodimer binds to fatty acids such as arachidonic acid, while neither monomer does. S100A9 and the heterodimer promote neutrophil infiltration and degranulation at sites of inflammation and inflammatory cytokine production by monocytes. S100A9 also promotes cartilage matrix destruction in osteoarthritis as well as RAGE-dependent cardiac fibrosis and remodeling. In the brain, the S100A9 homodimer promotes neuronal cytotoxicity, although this is reduced by its binding to amyloid-beta peptide. Post-translational: Phosphorylated. Phosphorylation inhibits activation of tubulin polymerization. S-nitrosylation of Cys-3 is implicated in LDL(ox)-induced S-nitrosylation of GAPDH at 'Cys-247' through a transnitrosylase mechanism involving a iNOS-S100A8/9 complex (PubMed:25417112). Methylation at His-105 by METTL9 reduces zinc-binding without affecting heterodimerization with S100A8.

Immobilized Recombinant Human S100A9 Protein (rp209720) at 1.0 μg/mL can bind Recombinant Human S100A8 Protein (rp183594) with the EC50 of 189.6 ng/mL.

重组表达

13.1 kDa

No tag

12.2 & 27.2 kDa, under reducing conditions; 12.2 & 27.2 kDa, under non-reducing conditions.

E. coli Accession #: P06702 | E. coli

<1.0 EU/μg

人(Human)

2-114 aa

TCKMSQLERNIETIINTFHQYSVKLGHPDTLNQGEFKELVRKDLQNFLKKENKNEKVIEHIMEDLDTNADKQLSFEEFIMLMARLTWASHEKMHEGDEGPGHHHKPGLGEGTP

No

Yes