Recombinant Human NRG1-alpha/HRG1-alpha Protein

重组人NRG1-alpha/HRG1-alpha蛋白 | 重组人神经调节蛋白1-α蛋白

ARIA | GGF2 | HGL | HRG | HRG1 | MST131 | MSTP131 | neuregulin 1 | Neuregulin1 alpha | Neuregulin-1 alpha | pro-neuregulin-1, membrane-bound isoform | pro-NRG1 | sensory and motor neuron derived factor | SMDF

rp216653-10μg

10μg

无动物源

≥90%(SDS-PAGE)

The neuregulin family of structurally related glycoproteins comprises products from four distinct but related genes, Nrg-1, Nrg-2, Nrg-3, and Nrg-4. Through alternative splicing or the use of alternative promoters, Nrg-1 encodes more than 14 soluble or transmembrane proteins. The extracellular domain of the transmembrane NRG1 isoforms can be proteolytically cleaved to release soluble growth factors. The alpha - or beta -splice variants differ in their C-terminal region. All NRG1 isoforms contain an EGF-like domain that is required for their direct binding to the ErbB3 or ErbB4 receptor tyrosine kinases. The ErbB3 or ErbB4 subsequently recruits and heterodimerizes with ErbB2, resulting in tyrosine phosphorylation and NRG1 signaling. NRG1 isoforms can be classified into three major subtypes. Type I (Neu Differentiation Factor, NDF; Heregulin, HRG; Acetylcholine Receptor Inducing Activity, ARIA) and type II (Glial Growth Factor, GGF). NRG1s have an immunoglobulin (Ig)-like domain N-terminal to the EGF-like domain. Type I NRG1s differ from type II NRG1s by having a glycosylation-rich domain between the Ig-like and the EGF-like domains. Type III NRG1 (Sensory and Motor neuron-Derived Factor) lacks the Ig-like domain but has a cysteine rich domain (CRD) instead. NRG1 isoforms show distinct spatial and temporal expression patterns. These proteins play important roles during development of both the nervous system and the heart. They have been shown to regulate the selective expression of neurotransmitter receptors in neurons and at the neuromuscular junction, and to promote the differentiation and development of Schwann cells from neural crest stem cells. NRG1s have also been shown to be involved in the establishment of the oligodendroglial lineage. Post-translational: Proteolytic cleavage close to the plasma membrane on the external face leads to the release of the soluble growth factor form. N- and O-glycosylated. Extensive glycosylation precedes the proteolytic cleavage (By similarity).

Measured in a cell proliferation assay using MCF‑7 human breast cancer cells. The ED₅₀ for this effect is 8.1 ng/mL.

重组表达

24.9 kDa

C-His

45.0-55.0 kDa, under reducing conditions

HEK293 Accession #: Q02297-1 | HEK293

<1.0 EU/μg

人(Human)

20-241 aa

MDAMKRGLCCVLLLCGAVFVSPSGKKPESAAGSQSPALPPRLKEMKSQESAAGSKLVLRCETSSEYSSLRFKWFKNGNELNRKNKPQNIKIQKKPGKSELRINKASLADSGEYMCKVISKLGNDSASANITIVESNEIITGMPASTEGAYVSSESPIRISVSTEGANTSSSTSTSTTGTSHLVKCAEKEKTFCVNGGECFMVKDLSNPSRYLCKCQPGFTGARCTENVPMKVQNQEKAEELYQKHHHHHH

Yes

Yes