Recombinant Human DNMT1 Protein

重组人DNMT1蛋白 | 重组人DNA甲基转移酶1蛋白

ADCADN | AIM | CXXC finger protein 9 | CXXC-type zinc finger protein 9 | CXXC9 | DNA (cytosine 5 ) methyltransferase 1 | DNA (cytosine-5)-methyltransferase 1 | DNA methyltransferase 1 | DNA methyltransferase HsaI | DNA methyltransferase M.HsaI | DNA MTase

rp217144-10μg

10μg

无载体

≥85%(SDS-PAGE)

Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Post-translational: Sumoylated; sumoylation increases activity.Acetylation on multiple lysines, mainly by KAT2B/PCAF, regulates cell cycle G2/M transition. Deacetylation of Lys-1349 and Lys-1415 by SIRT1 increases methyltransferase activity.Phosphorylation of Ser-154 by CDKs is important for enzymatic activity and protein stability. Phosphorylation of Ser-143 by AKT1 prevents methylation by SETD7 thereby increasing DNMT1 stability.Methylation at Lys-142 by SETD7 is necessary for the regulation of DNMT1 proteasomal degradation.Ubiquitinated by UHRF1; interaction with USP7 counteracts ubiquitination by UHRF1 by promoting deubiquitination and preventing degradation by the proteasome.

重组表达

24.3 kDa

N-His

26.8 kDa, under reducing conditions; 22.6 & 25.1 & 26.2 & 47.0 kDa, under non-reducing conditions.

E. coli Accession #: P26358 | E. coli

<1.0 EU/μg

人(Human)

1381-1587 aa

MHHHHHHASALEISYNGEPQSWFQRQLRGAQYQPILRDHICKDMSALVAARMRHIPLAPGSDWRDLPNIEVRLSDGTMARKLRYTHHDRKNGRSSSGALRGVCSCVEAGKACDPAARQFNTLIPWCLPHTGNRHNHWAGLYGRLEWDGFFSTTVTNPEPMGKQGRVLHPEQHRVVSVRECARSQGFPDTYRLFGNILDKHRQVGNAVPPPLAKA

No

Yes