Recombinant Human PCCB Protein

重组人PCCB蛋白 | 重组人线粒体丙酰辅酶A羧化酶β链蛋白

DKFZp451E113 | PCCase subunit beta | pccB | PCCB_HUMAN | pccBC Complementation group | Propanoyl CoA:carbon dioxide ligase subunit beta | Propanoyl-CoA:carbon dioxide ligase subunit beta | Propionyl CoA carboxylase beta chain | mitochondrial | propionyl C

rp217614-10μg

10μg

无载体

≥90%(SDS-PAGE)

This is one of the 2 subunits of the biotin-dependent propionyl-CoA carboxylase (PCC), a mitochondrial enzyme involved in the catabolism of odd chain fatty acids, branched-chain amino acids isoleucine, threonine, methionine, and valine and other metabolites (PubMed:15890657, PubMed:6765947). Propionyl-CoA carboxylase catalyzes the carboxylation of propionyl-CoA/propanoyl-CoA to D-methylmalonyl-CoA/(S)-methylmalonyl-CoA (PubMed:15890657, PubMed:6765947). Within the holoenzyme, the alpha subunit catalyzes the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain, while the beta subunit then transfers the carboxyl group from carboxylated biotin to propionyl-CoA (By similarity). Propionyl-CoA carboxylase also significantly acts on butyryl-CoA/butanoyl-CoA, which is converted to ethylmalonyl-CoA/(2S)-ethylmalonyl-CoA at a much lower rate (PubMed:6765947). Other alternative minor substrates include (2E)-butenoyl-CoA/crotonoyl-CoA (By similarity).

重组表达

33.9 kDa

N-His

31.1 & 69.1 kDa, under reducing conditions; 29.2 & 30.7 & 65.5 & 120.0 kDa, under non-reducing conditions.

E. coli Accession #: P05166 | E. coli

<1.0 EU/μg

人(Human)

240-539 aa

MHHHHHHTQEELGGAKTHTTMSGVAHRAFENDVDALCNLRDFFNYLPLSSQDPAPVRECHDPSDRLVPELDTIVPLESTKAYNMVDIIHSVVDEREFFEIMPNYAKNIIVGFARMNGRTVGIVGNQPKVASGCLDINSSVKGARFVRFCDAFNIPLITFVDVPGFLPGTAQEYGGIIRHGAKLLYAFAEATVPKVTVITRKAYGGAYDVMSSKHLCGDTNYAWPTAEIAVMGAKGAVEIIFKGHENVEAAQAEYIEKFANPFPAAVRGFVDDIIQPSSTRARICCDLDVLASKKVQRPWRKHANIPL

No

Yes