Recombinant Human BRAF Protein

重组人BRAF蛋白 | 重组人B-Raf原癌基因蛋白

FLJ95109 | 94 kDa B raf protein | B raf | B raf 1 | B Raf proto oncogene serine threonine protein kinase | B Raf proto oncogene, serine/threonine kinase | B RAF1 | B-Raf proto-oncogene serine/threonine-protein kinase (p94) | BRAF | BRAF 1 | BRAF_HUMAN | B

rp217617-10μg

10μg

无载体

≥95%(SDS-PAGE)

BRAF gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. BRAFprotein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in BRAF gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in BRAF gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. Post-translational: Phosphorylation at Ser-365 by SGK1 inhibits its activity (Ref.8, PubMed:11410590). Phosphorylation at Thr-753 by MAPK1 (PubMed:19710016). Dephosphorylation of Ser-365 by the SHOC2-MRAS-PP1c (SMP) complex consisting of SHOC2, GTP-bound M-Ras/MRAS and the catalytic subunit of protein phosphatase 1 (PPP1CA, PPP1CB or PPP1CC); this relieves inactivation and stimulates kinase activity (PubMed:35768504, PubMed:35830882). Methylation at Arg-671 decreases stability and kinase activity. Ubiquitinated by RNF149; which leads to proteasomal degradation. Polyubiquitinated at Lys-578 in response to EGF.

重组表达

22.2 kDa

N-His

24.2 kDa, under reducing conditions; 24.8 & 46.0 kDa, under non-reducing conditions.

E. coli Accession #: P15056 | E. coli

<1.0 EU/μg

人(Human)

49-239 aa

MHHHHHHNIKQMIKLTQEHIEALLDKFGGEHNPPSIYLEAYEEYTSKLDALQQREQQLLESLGNGTDFSVSSSASMDTVTSSSSSSLSVLPSSLSVFQNPTDVARSNPKSPQKPIVRVFLPNKQRTVVPARCGVTVRDSLKKALMMRGLIPECCAVYRIQDGEKKPIGWDTDISWLTGEELHVEVLENVPLTTHNFVR​

No

Yes