Recombinant Human CYP24A1 Protein

重组人 CYP24A1 蛋白 | 重组人1 | 25-二羟基维生素D₃ 24-羟化酶

25-dihydroxyvitamin D(3) 24-hydroxylase | mitochondrial | EC:1.14.15.16 | 24-OHase | Vitamin D(3) 24-hydroxylase | Cytochrome P450 24A1 | Cytochrome P450-CC24 | CYP24A1 | CYP24

rp220434-10μg

10μg

无载体

≥95%(SDS-PAGE)

A cytochrome P450 monooxygenase with a key role in vitamin D catabolism and calcium homeostasis. Via C24- and C23-oxidation pathways, catalyzes the inactivation of both the vitamin D precursor calcidiol (25-hydroxyvitamin D3) and the active hormone calcitriol (1-alpha,25-dihydroxyvitamin D3) (PubMed:11012668, PubMed:15574355, PubMed:16617161, PubMed:24893882, PubMed:29461981, PubMed:8679605). With initial hydroxylation at C-24 (via C24-oxidation pathway), performs a sequential 6-step oxidation of calcitriol leading to the formation of the biliary metabolite calcitroic acid (PubMed:15574355, PubMed:24893882). With initial hydroxylation at C-23 (via C23-oxidation pathway), catalyzes sequential oxidation of calcidiol leading to the formation of 25(OH)D3-26,23-lactone as end product (PubMed:11012668, PubMed:8679605). Preferentially hydroxylates at C-25 other vitamin D active metabolites, such as CYP11A1-derived secosteroids 20S-hydroxycholecalciferol and 20S,23-dihydroxycholecalciferol (PubMed:25727742). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via FDXR/adrenodoxin reductase and FDX1/adrenodoxin (PubMed:8679605).

重组表达

12.5 kDa

N-His

12.5 & 24.5 kDa, under reducing conditions; 15.1 & 25.1 & 31.1 & 41.5 kDa, under non-reducing conditions.

E. coli Accession #: Q07973 | E. coli

<1.0 EU/μg

人(Human)

415-514 aa

MHHHHHHLMLNTQVLGSSEDNFEDSSQFRPERWLQEKEKINPFAHLPFGVGKRMCIGRRLAELQLHLALCWIVRKYDIQATDNEPVEMLHSGTLVPSRELPIAFCQR

No

Yes